Two scientists at the Air Force Research Laboratory have invented a novel method for detecting biofouling in fuel tanks. Businesses can make, use, or sell the technology after negotiating a patent license agreement.
Bio-contamination can cause a myriad of problems in a fuel system including, hydrocarbon degradation, changes in fuel properties and quality, corrosion, filter clogging, deactivation of fuel-water coalescers, coating degradation, inaccurate fuel level readings, decreased vehicle performance.
Early detection of biofouling enables the use of cost-effective mitigation strategies that may reduce the contamination’s impact on the fuel system. An early warning detection sensor to alert maintenance crew of bio-contamination could save millions of dollars per year in repair costs over the lifetime of the vehicle and fuel system.
To date, there has been no simple and reliable method for detecting microbes and bio-deterioration in fuel. The methods used today are typically performed by highly trained scientists in laboratories equipped with molecular-based instrumentation that are quantitative in nature and do not differentiate between living and non-living microbes. While more straightforward colony counting methods are quantitative and do not require expensive instrumentation this approach is time-consuming and only capable of detecting culturable bacteria, which may represent just 10% of all bacteria present within a fuel system.
Commercially available detection kits can be cumbersome, inaccurate, and, at best, semi-quantitative. Some of these kits require long culture growth for visual analysis or quantification of Adenosine Triphosphate (ATP). However, ATP levels are highly dependent on the growth stage of the organism. Other commercially available kits use antibody-based detection methods which are affected by degradation and are negatively influenced by the presence of fuel.
In their newly patented process, Air Force scientists Oscar Ruiz and Oksana Pavlyuk use broad-range peptide biorecognition elements that target cell surface determinants produced by hydrocarbon-degrading microorganisms during growth in the fuel. The biorecognition elements are cross-linked to quantum dots and a reporter molecule that can generate a chemiluminescent, fluorescent, colorimetric or transducing nanomaterial signal. Biorecognition elements provide several benefits over other molecular probes, such as high chemical diversity, ease of synthesis and conjugation to the surface of a signal transducer, and high stability in harsh environments, such as fuel.
- Quick, reliable, and accurate detection of bio-contamination within fuel systems
- Minimizes repair and replacement costs of equipment due to bio-deterioration
- Businesses can commercialize the technology by licensing U.S. Patent 10,295,537 from the Air Force
- Patent license fees paid to the Air Force are negotiable
- TechLink guides businesses through evaluation and licensing; services provided at no cost
- Companies that license the technology may potentially collaborate with Air Force researchers