Navy

NRL Sheath Flow – Microflow Cytometer

Compact, robust, low power consumption, high-performance cytometer

Medical & Biotechnology

The Naval Research Laboratory (NRL) has pioneered and advanced a technique in controlling flow at the micro-scale: NRL sheath flow.

It uses one or more shapes (grooves, chevrons, et cetera) placed in the top and bottom of the channel. The shapes direct the sheath fluid around the core stream, separate the core stream from the walls of the channel and sculpt material distribution (Fig.1).

The high flow rate of the sheath fluid compared to the sample stream focuses the sample into a very narrow-diameter core that is exposed to the wider laser beams in the interrogation region of the cytometer (Fig.2). This revolutionary system uses static geometric features to sculpt material distributions. The system utilizes a core stream and one or more sheath streams that are introduced into a single channel.

An exemplary device (depicted in Fig. 3) was used to demonstrate multiplexed detection of bacteria and toxins using fluorescent coded microspheres. Anti-body-coated microspheres bound biothreat targets in a sandwich immunoassay format. The MFC focused the microspheres in three dimensions within the laser interrogation regions using the passive groove structure. Optical analysis at four different wavelengths identified the coded microspheres and quantified target bound by the presence of phycoerythrin tracer. The multiplexed assays in the MFC had performance approaching that of a commercial benchtop unit.

Combining NRL sheath flow designs and integrated waveguides for optical interrogation, NRL has built MFCs for various analytes addressing problems in environmental monitoring to those with clinical interests. One unit characterized and discriminated between populations of phytoplankton and was deployed in an unmanned vehicle. Sheath fluids in NRL MFCs can be recaptured and reused.

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